Composite

Part:BBa_K3120019

Designed by: Shuiqin Gong   Group: iGEM19_BM-AMU   (2019-08-14)


5LTR+TRE+pCAG+Snail1+IRES+NeoR+3LTR+AmpR

The main function of this part is to act as an induction expression system. In the presence of DOX, tetR-Krab binds with TRE, thus promoting the transcription of downstream snail1 and promoting the EMT process.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 20
    Illegal BglII site found at 3300
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2
    Illegal BsaI site found at 3282
    Illegal BsaI site found at 4183
    Illegal SapI.rc site found at 2955
    Illegal SapI.rc site found at 3165


[edit]
Categories
Parameters
None